Regulatory

Part:BBa_K2272001:Design

Designed by: Eric Joshua Wang   Group: iGEM17_Virginia   (2017-10-21)


promoter MnSOD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As pMnSOD is only 133 b.p. in length, a denser gel should be used to perform gel electrophoresis for this promoter. In our case, we used Nusieve agarose gel for pMnSOD.


Source

Hypoxic modulation of manganese superoxide dismutase promoter activity and gene expression in lung epithelial cells. https://www.ncbi.nlm.nih.gov/pubmed/10385600

MnSOD in Oxidative Stress Response-Potential Regulation via Mitochondrial Protein Influx. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3942709/#B127

References